Park et al. present the crystal structure of an RPGR–TTLL5 co-complex, which reveals TTLL recognition paradigms for non-tubulin substrates and sheds light on disease mechanism for retinal dystrophies caused by TTLL5 and RPGR mutations.

Yu et al. reveal how regional transcription factors control the morphology of enteroendocrine cells in fruit flies. Their findings demonstrate that a Ptx1-esg-sSJ regulatory axis determines whether enteroendocrine cells integrate into the gut epithelium, thereby influencing their ability to sense dietary nutrients.

Neurons rely on molecular motors to deliver proteins to precise locations. This study shows that kinesin-2 motors form heterogeneous assemblies with distinct cargo preferences. A KIF3B-enriched assembly preferentially associates with TRIM46, suggesting that variation in motor composition contributes to selective transport during neuronal development.

Shen et al. demonstrate that LMNA reduction alters peripheral genome organization in human cardiomyocytes through cytoskeletal forces transmitted by the LINC complex. These findings uncover a microtubule-dependent pathway regulating lamina-associated domains and suggest that the cytoskeleton may contribute to early nuclear dysfunction in laminopathies.

Agarwal et al. define the stage-specific gene expression programs of a leader cell that drives collective tissue invasion during organ development. By combining transcriptomics with functional analysis, they identify membrane trafficking as a central regulator of leader cell behavior, providing a valuable resource for studying collective cell migration in vivo.

Deb Roy et al. introduce an optogenetic tool to rapidly induce microtubule acetylation in living cells. The study shows that microtubule acetylation directly triggers release of the RhoGEF GEF-H1 from microtubules, activating actomyosin contractility, promoting focal adhesion maturation, and driving persistent directional cell migration.

Stalder and Pereira et al. combine affinity isolation of post-Golgi carriers, mass spectrometry, and a pooled CRISPR-KO screen to uncover new regulators of Golgi-to-plasma membrane transport. They show that a PTPN23-dependent ESCRT pathway is essential for the secretion of membrane and soluble cargoes, including hormones and antibodies, in specialized cells.